Application of 17α-methyltestosterone to enhance broodstock maturation of the domesticated black tiger shrimp (Penaeus monodon): A comparative study of injection method and oral method

Abstract The effects of 17α-methyltestosterone administration on spermatophore quality in captive broodstock tiger shrimp Penaeus monodon were investigated using injection method and oral based the optimized protocol. experiment was designed to compare group with two replications during a 38-day experimental period. Captive male weighing 57-95 g collected from ponds stored 10,000 m 3 concrete tank (20 shrimp/tank). Male broodstocks injected orally administrated at dose 300 ng/100 body weight. Data indicates that showed percentage mature count normal spermatozoa 1.07 1.38% higher significantly differs through mixing feed maturation stage. However, time 1 st re-maturation both methods, weight spermatophores number did not show any difference. Based comparison methods used, results are obtained hormone can be done injection, but hormones gives better than matures first simultaneously.